Chemical Communication (2022): “Novel triple mutant of an extremophilic glycosyl hydrolase enables the rapid synthesis of thioglycosides”
In order to expand the toolbox of enzymes available for thioglycoside synthesis, we describe here the first example of an extremophilic glycosyl hydrolase from Halothermothrix orenii (HorGH1) engineered towards thioglycosynthase activity with a novel combination of mutations. Using the triple mutant, HorGH1 M299R/E166A/E354G, a range of thioglycosides from glycosyl fluoride donors and aromatic thiols could be synthesised with […]
J Biotech (2021): “Producing natural vanilla extract from green vanilla beans using a β-glucosidase from Alicyclobacillus acidiphilus”
Last in the trilogy of Lidia’s PhD, lovely work completed with the help of Chris where a very rare green vanilla pod was used as the substrate! The paper was accepted just before Lidia’s Phd viva, a good sign! Read the full paper here Related Posts
ChemBioChem (2021): “Release of soybean isoflavones using a β-Glucosidase from Alicyclobacillus herbarius”
Well done to Lidia and Chris and massive thank you to Louise Gourlay (excellent crystallographer at the University of Milan) for this great work on the extraction of isofavones from soy flour with a new glycosyl hydrolase. Abstract: β-Glucosidases are used in the food industry to hydrolyse glycosidic bonds in complex sugars, with enzymes sourced […]
Food Chemistry (2020): “Performance of the extremophilic enzyme BglA in the hydrolysis of two aroma glucosides in a range of model and real wines and grape juices”
Very well done Lidia on successfully completing this challenging project! A very good results from the time spent in Australia, and for sure finding new ways to improve aromaticity in wines is ideal at the time of this quarantine…Here we have exploited an extremophilic glycosyl hydrolases which is showing exceptional tolerance to high concentrations of […]
Appl Microbiol Biotechnol (2020): “Engineering novel S-glycosidase activity into extremo-adapted β-glucosidase by rational design”
This paper presents Nourah’s work on how strategic site directed mutagenesis has been successfully applied to shift substrate preference in glycosyl hydrolases. The mutant enzyme is now able to work with thio-glycosides which are found in several plants and have shown both antimicrobial and anticancer activity. In fact the identified positions could be used also […]