Chemical Communication (2022): “Novel triple mutant of an extremophilic glycosyl hydrolase enables the rapid synthesis of thioglycosides”
In order to expand the toolbox of enzymes available for thioglycoside synthesis, we describe here the first example of an extremophilic glycosyl hydrolase from Halothermothrix orenii (HorGH1) engineered towards thioglycosynthase activity with a novel combination of mutations. Using the triple mutant, HorGH1 M299R/E166A/E354G, a range of thioglycosides from glycosyl fluoride donors and aromatic thiols could be synthesised with […]
ACS Sustainable Chemistry and Engineering (2021): ” Enantio-complementary continuous-flow synthesis of 2-aminobutane using covalently immobilized transaminases”
Amazing work with our collaborator Dr. Beatriz Domingues in Johnson Matthey. Chris engineered our evergreen HeWT to make it enantioselective in the synthesis of the smallest chiral amine, 2 amino butane. Well done! You can find the paper here Related Posts